BURKITTS-LYMPHOMA CELL-LINE BEARING SURFACE IGA AND NEGATIVE FOR NUCLEAR ANTIGEN OF EPSTEIN-BARR VIRUS (EBNA)

  • 1 January 1980
    • journal article
    • research article
    • Vol. 50  (6) , 423-434
Abstract
A new continuous cell line, TL-1, was established in tissue culture from Burkitt''s lymphoma arising at the ileocecal region [in a human]. It was maintained for 2 yr. The cells were small and round and grew in a suspension culture. Population doubling time was .apprx. 36 h. The cells revealed the chromosomal abnormality, i.e., translocation between 8 and 14 chromosomes, reported in some Burkitt''s lymphomas. This line was negative for nuclear antigen of EB virus (EBNA) and bore surface IgA but no receptors. Inoculums of 107 cells produced tumors in the athymic nude mice. The histological appearance of the tumor was consistent with Burkitt''s lymphoma. Ultrastructural findings showed nuclear blebs and clefts.