Generation of Recombinant Vaccinia Viruses
Open Access
- 1 May 1998
- journal article
- unit
- Published by Wiley in Current Protocols in Protein Science
- Vol. 13 (1) , 5.13.1-5.13.19
- https://doi.org/10.1002/0471140864.ps0513s13
Abstract
This unit first describes how to infect cells with vaccinia virus and then transfect them with a plasmid‐transfer vector to generate a recombinant virus. Methods are also presented for purifying vaccinia virus and for isolating viral DNA, which can be used during transfection. Also presented are selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented.Keywords
This publication has 22 references indexed in Scilit:
- Host Range and Cytopathogenicity of the Highly Attenuated MVA Strain of Vaccinia Virus: Propagation and Generation of Recombinant Viruses in a Nonhuman Mammalian Cell LineVirology, 1997
- Construction and Characterization of Vaccinia Direct Ligation VectorsVirology, 1997
- Development of a replication-deficient recombinant vaccinia virus vaccine effective against parainfluenza virus 3 infection in an animal modelVaccine, 1996
- Evaluation of the thymidine kinase (tk) locus as an insertion site in the highly attenuated vaccinia MVA strainArchiv für die gesamte Virusforschung, 1996
- A novel vaccinia virus expression system allowing construction of recombinants without the need for selection markers, plasmids and bacterial hostsJournal of General Virology, 1995
- Selection of recombinant vaccinia viruses on the basis of plaque formationGene, 1995
- A recombinant vector derived from the host range-restricted and highly attenuated MVA strain of vaccinia virus stimulates protective immunity in mice to influenza virusVaccine, 1994
- Mapping of deletions in the genome of the highly attenuated vaccinia virus MVA and their influence on virulenceJournal of General Virology, 1991
- Reverse guanine phosphoribosyltransferase selection of recombinant vaccinia virusesVirology, 1990
- Reviews : Video in Mental Health Practice IRA HEILWEIL Tavistock Publications 1984, pb; pp 186; £4.95Probation Journal, 1984