Effect of 5-Fluoro-2′-deoxyuridine on Deoxyribonucleotide Pools In Vivo2
- 1 September 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 65 (3) , 603-605
- https://doi.org/10.1093/jnci/65.3.603
Abstract
5-Fluoro-2′-deoxyuridine (FdUrd) lowered the dTTP levels in rapidly frozen 12-day W rat embryos and in a human neuroblastoma grown in nude N:NIH(S) mice to about 20% of control values. This effect was associated with greatly increased dGTP levels and reduction of dGTP levels essentially to zero. Elimination of the dGTP pool correlated temporally with the cytotoxicity of FdUrd. Extremely rapid fixation of tissue was required to avoid artifactually high deoxyribonucleoside triphosphate values.Keywords
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