The Effect of Ionizing Radiation on a MarinePseudomonasSp.

Abstract
The effect of ionizing radiation on a marine Pseudomonas sp. has been tested under the following experimental conditions: Unirradiated cells inoculated into irradiated media; irradiated cells plated immediately after irradiation; irradiated cells incubated in irradiated media. In this study irradiated liquid synthetic medium was found to have a bactericidal effect on a radiosensitive marine Pseudomonas sp. taken from exponentially-growtin cultures. These indirect toxic effects of the irradiated media were, however, relatively small compared with the total effect of direct irradiation of the organisms in suspensions. The indirect toxic effects of the medium were found to decrease slowly with time. They were assumed to be due mainly to the combination of glucose and ammonium chloride in the medium. The turbidimetric method used was found to be time- saving and gave accurate results. It can thus be recommended for similar investigations.

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