THE RÔLE OF THE GLOMERULI AS THE PREFERENTIAL ROUTE FOR EXCRETION OF PHENOLSULPHONEPHTHALEIN IN THE FROG'S KIDNEY

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Abstract
Injection of the glomeruli through the renal portal vein in the frog is easily accomplished, and, on an average, shows about a 30% glomerular injection in the whole kidney, in some cases reaching twice that figure. The observation that phenolsulphonephthalein, when added to the renal portal blood or perfusate, is eliminated in the urine, as first demonstrated by Rowntree and Geraghty, is confirmed. The mechanism of this elimination parallels the accessibility of the glomeruli to renal portal blood, and it is absent when the renal portal blood does not enter the glomeruli, as shown by simultaneous renal portal india ink injections. The accessibility of the tubular capillaries to the renal portal blood flow appears to have no marked relation to the excretion of phenolsulphonephthalein injected as described above. A new technique for the study of kidney function in the frog is described, by which substances injected into 1 or the other blood supply can then reach more or less of the glomeruli in the 2 halves of the kidney and thus result in varying secretions. The frog''s kidney submitted to this procedure is able to concentrate phenolsulphonephthalein at least for 15-20 min., thus indicating that the kidney is functioning in a manner close to normal during this time. These results tend to confirm the conception of Nussbaum, that in the tubular capillaries a complete mixing of renal arterial and renal portal venous blood occurs, and it is still further confirmed by Richards and his co-workers (1927-28). These results also confirm Adami''s criticism of Nuss-baum''s experiment; namely, that after ligation of the renal arteries in the frog, the glomeruli may still obtain blood, probably from the renal portal vein. Glomerular elimination of phenolsulphonephthalein in the frog''s kidney appears to be the method of preferential excretion.

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