Separation of Cobalt (III) Bis(ethylenediamine) Amino Acid Complexes by Reversed Phase HPLC
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 4 (4) , 689-700
- https://doi.org/10.1080/01483918108059965
Abstract
Methods for the rapid analysis of amino acid cobalt (III) bis(ethylenediamine) complexes by reversed phase high performance liquid chromatography (HPLC) are described with mobile phases containing the pairing ions, p-toluenesulfonate and hexanesulfonate. Under these conditions the amino acid cobalt (III) bis(ethylenediamine) complexes elute in order of the relative hydrophobicities of the parent amino acids which suggests that the amino acid side chain make a significant contribution to the retention mechanism. At high sample loadings, these complexes show a concentration dependent peak splitting effect divergent to that normally experienced with inadequate buffering capacity of the pairing ion reagent.This publication has 17 references indexed in Scilit:
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