Some Factors Affecting the Determination of Plasma Protein-Bound Iodine, Using the Alkaline Fusion–Ceric Sulfate Method

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Abstract
Effects of length and temperature of storage of plasma on the determination of plasma protein-bound iodine (PBI) by the alkaline fusion-ceric sulfate method were studied. Additional information was gained on the other method error term variances and on the derived standard error (S.E.) and coefficient of variation (C.V.), as a function of one or more fusions per plasma sample and one or more photometric determinations per fusion tube. Direct effects due to length and temperature of storage of plasma contributed little to the total variance. Interactions of length and temperature of storage with each other and animal (i.e., handling effects) constituted 25% of the total variance and may be eliminated by processing and assaying plasma samples immediately after blood collection. Based on method error term variance the standard error of a single plasma PBI determination was 0.46 [mu]g % and the coefficient of variation was 8.8%. The most effective method of reducing these values was to increase the number of fusion tubes per plasma sample.

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