Determination of glucose level needed to induce aflatoxin production in Aspergillus parasiticus

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Abstract
Previous studies have established that aflatoxin production is induced by high levels of glucose (or other compatible carbohydrates). However, the minimal amount of glucose needed to achieve this effect had not been determined. Aspergillus parasiticus was induced to produce aflatoxin when incubated for 18 h in the presence of ≥ 0.1 M glucose before addition of cycloheximide. Toxin formation was not induced at any glucose concentration in the mycelia incubated for 8 h. These results suggest that aflatoxin synthesis would not be expected in substrate containing < 0.1 M of a carbohydrate source.