The Isolation of Crystalline Trypsinogen and Its Conversion into Crystalline Trypsin
- 30 November 1934
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 80 (2083) , 505-506
- https://doi.org/10.1126/science.80.2083.505
Abstract
Inactive trypsin, trypsinogen) obtained from an acid extract of fresh cattle nancreas crystallized in the form of short, triangular, prisms if allowed to stand at 5[degree]C. in 1/2 sat. MgSO4 made up in 0.2 M borate buffer pH 9.0. The crystalline trypsinogen was changed into crystalline trypsin when redissolved in dilute acid and then allowed to stand in 1/2 sat. MgSO4 pH 7.0-8.0 at 5[degree]C. Trypsin crystallized in the form of rectangular long prisms.This publication has 4 references indexed in Scilit:
- CRYSTALLINE CHYMO-TRYPSIN AND CHYMO-TRYPSINOGENThe Journal of general physiology, 1935
- AUTOCATALYTIC ACTIVATION OF TRYPSINOGEN IN THE PRESENCE OF CONCENTRATED AMMONIUM OR MAGNESIUM SULFATEScience, 1934
- Autocatalytic Activation of Trypsinogen in the Presence of Concentrated Ammonium or Magnesium SulfateScience, 1934
- Isolation of a Crystalline Protein from Pancreas and Its Conversion into a New Crystalline Proteolytic Enzyme by TrypsinScience, 1933