Differentiation of Acetomonas and Pseudomonas

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Abstract
A comparative study of 50 strains of Acetomonas and 165 strains of Pseudomonas showed that the two genera could be easily and rapidly differentiated by the use of four criteria; these were: (a) production of acid on ethanol+CaCO3 agar plates, (b) oxidation of calcium lactate to carbonate, (c) production and accumulation of dihydroxyacetone in glycerol media, (d) growth at (initial) pH 4.5. Acetomonas was positive with (a), (c) and (d), but negative with (b); Pseudomonas was positive only with (b). Liquefaction of gelatin and production of a greenish yellow fluorescent pigment, being variable with Pseudomonas and absent from Acetomonas, were useful only when positive.

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