Detection of Candida albicans mannan by immunodiffusion, counterimmunoelectrophoresis, and enzyme-linked immunoassay

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Abstract
Anti-mannan was produced in rabbits after peptidoglucomannan in adjuvant was injected. The antiserum was used to detect mannan by immunodiffusion and counterimmunoelectrophoresis (CIE) in gel and by sandwich enzyme-linked immunosorbent assay (ELISA). The antiserum detected lower concentrations of mannan of serotype A than of serotype B. Except in CIE, the reactions were more pronounced at 4°C than at higher temperatures. CIE detected 0.8 μg/ml mannan A or 12.5 μ/ml mannan B. Sandwich ELISA detected 3 ng/ml mannan A or 105 ng/ml mannan B. Mannan was not detected in the serum of patients or rabbits with candidiasis.