Analysis and γ-Irradiation of Polymer-Bound Catalase

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Abstract
Preliminary results are given for a pyrolysis-gas chromatographic technique for estimating the enzyme content of insoluble, polymer-enzyme conjugates. Samples of poly(diazostyrene)-bound catalase having known catalase contents were irradiated with 60Co γ-rays at pH 7 in dilute, oxygenated, aqueous suspensions. Indices of radiation protection showed that the conjugation to insoluble polymer protected the en2yme from the effects of radiation more effectively than diglycylglycine. This protection is interpreted in terms of radical scavenging and the physical structure of the polymer-catalase conjugate.