Determination of the endogenous phosphorylation state of B‐50/GAP‐43 and neurogranin in different brain regions by electrospray mass spectrometry
- 8 July 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 389 (3) , 309-313
- https://doi.org/10.1016/0014-5793(96)00612-6
Abstract
Electrospray mass spectrometry coupled to liquid chromatography was utilized to measure two PKC neuronal substrates, B-50/GAP-43 and neurogranin, in single rat brain areas. Aliquots of perchloric acid extracts were directly injected and mass spectra recorded. At elution times of 14.2 and 27.0 min two molecular species of MW 7450 and 23 602 Da were observed. These values are in excellent agreement for the expected MW for rat neurogranin and B-50/GAP-43. The presence of molecular species shifted by 80 mass units in both cases indicates that these proteins are present in phosphorylated forms in cortical and hippocampal extracts.Keywords
This publication has 22 references indexed in Scilit:
- Temporal Differences in the Phosphorylation State of Pre- and Postsynaptic Protein Kinase C Substrates B-50/GAP-43 and Neurogranin during Long Term PotentiationPublished by Elsevier ,1995
- Phosphorylation of the presynaptic protein B-50 (GAP-43) is increased during electrically induced long-term potentiationNeuron, 1992
- Measurement of relative amounts of phospho‐ and dephospho‐B‐50(GAP‐43) peptides by fast atom bombardment‐mass spectrometryFEBS Letters, 1992
- Role of the growth-associated protein B-50/GAP-43 in neuronal plasticityMolecular Neurobiology, 1991
- Monoclonal antibodies show that kinase C phosphorylation of GAP-43 during axonogenesis is both spatially and temporally restricted in vivo.The Journal of cell biology, 1991
- Subtractive cDNA cloning of RC3, a rodent cortex‐enriched mRNA encoding a novel 78 residue proteinJournal of Neuroscience Research, 1990
- Evidence for a Single Protein Kinase C‐Mediated Phosphorylation Site in Rat Brain Protein B‐50Journal of Neurochemistry, 1989
- Determination of Changes in the Phosphorylation State of the Neuron‐Specific Protein Kinase C Substrate B‐50 (GAP43) by Quantitative ImmunoprecipitationJournal of Neurochemistry, 1989
- Protein Phosphorylation and Neuronal FunctionJournal of Neurochemistry, 1985
- Presynaptic localization of B-50 phosphoprotein: the (ACTH)-sensitive protein kinase substrate involved in rat brain polyphosphoinositide metabolismBrain Research, 1985