Platelet Concentrates in an Additive Solution Prepared from Pooled Buffy Coats

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Abstract
Leukocyte-depleted platelet concentrates were prepared from pools of 4 buffy coats on the day after blood collection (BC-PC). The storage medium was composed of citrate phosphate dextrose plasma and a platelet-additive solution. Autologous transfusions of 111In-labelled platelets in 9 healthy volunteers were performed on the day of preparation (day 1) and on day 5. The recovery was 54.6±8.7 (day 1) and 51.9±10.4% (day 5), T1/2 was 101±28 and 61±9 h, respectively. The survival was 8.3±1.7 and 5.7±1.0 days, respectively, using linear plot, and 7.8±2.0 and 5.8±0.5 days using the multiple hit method. In a prospective clinical study a comparison of the corrected posttransfusion increments was made between BC-PCs and apheresis-PCs, and between fresh (1–2 days) and stored (3–5 days) preparations. No difference was found between BC-PCs and apheresis PCs. However, fresh BC-PCs gave higher increments than stored BC-PCs. A slight numerical difference between fresh and stored apheresis-PCs was not statistically significant. It is concluded that the BC-PC method results in platelets of equal quality to apheresis-PC.