Cell-Free Synthesis of Encephalomyocarditis Virus
- 1 June 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (11) , 6551-6555
- https://doi.org/10.1128/jvi.77.11.6551-6555.2003
Abstract
We developed a system for complete replication of encephalomyocarditis virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient virus synthesis occurred in a narrow range of Mg 2+ and EMCV RNA concentrations. Excess input RNA impaired RNA replication and virus production but not translation. This suggests the existence of a negative-feedback mechanism for regulation of RNA replication by the viral plus-strand RNA or proteins.Keywords
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