A meiotic gene conversion gradient opposite to the direction of transcription
- 1 September 1992
- journal article
- Published by Springer Nature in Nature
- Vol. 359 (6391) , 154-155
- https://doi.org/10.1038/359154a0
Abstract
Genetic recombination involves classical crossing-over and gene conversion (aberrant segregation). In fungi that produce an ascus containing four spores, a gene conversion event is manifested as 3:1 or 1:3 (or more rarely 4:0 or 0:4) segregations, in contrast to the normal mendelian 2:2 segregation. Polarity is one of the properties of gene conversion; in almost all cases the frequency of conversion exhibits a gradient across the gene monitored. The frequency of conversion is usually independent of the specific allele used as a marker, but dependent on its location. An interpretation of conversion polarity is that it is caused by the existence of specific initiation sites for meiotic recombination, located at the high end of the polarity gradient. Here we show that the polarity gradient for the HIS2 gene of Saccharomyces cerevisiae is high at the 3' end of the gene, implying that the promoter of HIS2 is not the initiation site.Keywords
This publication has 9 references indexed in Scilit:
- A promoter deletion reduces the rate of mitotic, but not meiotic, recombination at the HIS4 locus in yeastCurrent Genetics, 1992
- DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast.Proceedings of the National Academy of Sciences, 1991
- A poly(dA.dT) tract is a component of the recombination initiation site at the ARG4 locus in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1991
- [19] Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeastPublished by Elsevier ,1991
- An initiation site for meiotic gene conversion in the yeast Saccharomyces cerevisiaeNature, 1989
- Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast.Proceedings of the National Academy of Sciences, 1985
- Replacement of chromosome segments with altered DNA sequences constructed in vitro.Proceedings of the National Academy of Sciences, 1979
- Meiotic Gene Conversion: A Signal of the Basic Recombination Event in YeastCold Spring Harbor Symposia on Quantitative Biology, 1979
- GENE CONVERSION AND INTRAGENIC RECOMBINATION AT AT THE SUP6 LOCUS AND THE SURROUNDING REGION IN SACCHAROMYCES CEREVISIAEGenetics, 1976