Partial rapid metabolism of 1,2‐dichloroethane by Methylosinus trichosporium OB‐3b
- 1 April 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in Environmental Toxicology and Chemistry
- Vol. 11 (4) , 497-501
- https://doi.org/10.1002/etc.5620110407
Abstract
Resting cell suspensions of the soil methylotroph Methylosinus trichosporium OB‐3b rapidly dehalogenate ethylene dichloride, but the process stops after one halogen is removed. Work with 14C‐ and 13C‐labeled 1,2‐dichloroethane shows that the biodehalogenation entails a direct hydrox‐ylation of one of the CCl bonds to produce chloroethanol. The latter is rapidly oxidized to chloroacetic acid. The organism does not metabolize this acid on the time scale of these experiments.Keywords
This publication has 28 references indexed in Scilit:
- Biodehalogenation: The metabolism of vinyl chloride by Methylosinus trichosporium OB‐3b. A sequential oxidative and reductive pathway through chloroethylene oxideEnvironmental Toxicology and Chemistry, 1992
- Biodehalogenation: Oxidative and reductive metabolism of 1,1,2‐trichloroethane by Pseudomonas Putida—Biogeneration of vinyl chlorideEnvironmental Toxicology and Chemistry, 1990
- Methane monooxygenase catalyzed oxygenation of 1,1-dimethylcyclopropane. Evidence for radical and carbocationic intermediatesBiochemistry, 1990
- Biodehalogenation: Active site versus enzymatic and whole cell rates with cytochromes P‐450Environmental Toxicology and Chemistry, 1989
- Involvement of a Quinoprotein Alcohol Dehydrogenase and an NAD-dependent Aldehyde Dehydrogenase in 2-Chloroethanol Metabolism in Xanthobacter autotrophicus GJ10Microbiology, 1987
- Microbial dehalogenation of haloalkanes mediated by oxygenase or halidohydrolase.Agricultural and Biological Chemistry, 1986
- Metabolic Disposition Study of Chlorinated Hydrocarbons in Rats and MiceDrug and Chemical Toxicology, 1985
- In vivo 13C NMR Investigations of Methanol Oxidation by the Obligate Methanotroph Methylosinus trichosporium OB3bMicrobiology, 1984
- Oxidation of heme proteins by alkyl halides: a probe for axial inner sphere redox capacity in solution and in whole cellsBiochemistry, 1978
- Biodehalogenation. Metabolism of the nematocides cis- and trans-3-chloroallyl alcohol by a bacterium isolated from soilJournal of Agricultural and Food Chemistry, 1971