Hallucinogenic Species in Amanita Muscaria. Determination of Muscimol and Ibotenic Acid by Ion-Interaction HPLC
- 1 February 1997
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography & Related Technologies
- Vol. 20 (3) , 413-424
- https://doi.org/10.1080/10826079708010660
Abstract
Ibotenic acid and muscimol are considered the principal components responsible of the hallucinogenic properties of Amanita muscaria. In this paper a sensitive ion-interaction HPLC method is presented which permits the simultaneous determination of ibotenic acid and muscimol. A C18 reverse phase is the stationary phase and the mobile phase is an aqueous solution of 5.0 mM octylammonium o-phosphate. A spectrophotometric comparative detection performed at 230 and 254 nm is employed. Detection limits are of 18 μg/L for ibotenic acid and of 30 μg/L for muscimol.Keywords
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