Abstract
Trials utilizing 3 fistulated steers, in 2 Latin-square designed experiments, were conducted with 5 rations equal in digestible protein and containing cottonseed hulls, plus one of the following protein supplements: soybean meal, raw soybeans, corn gluten feed, urea, or urea + soybean meal. In the 1st experiment, soybean meal, raw soybeans, and corn gluten feed were fed. The corn gluten feed ration was associated with the highest concentrations of total N and protein N in the rumen fluid; the ration of raw soybeans was associated with the lowest concentrations; and the soybean meal ration with intermediate concentrations. Concentrations of ammonia-N were highest with soybean meal and the lowest with raw soybeans. Total volatile fatty acids (VFA) were similar for soybean meal and corn gluten feed. Soybean meal, urea, and urea + soybean meal were fed in the 2nd experiment. The soybean meal ration was associated with the highest concentrations of total N and protein N; the urea ration with the lowest concentrations; and the urea + soybean meal ration with intermediate concentrations. Concentrations of ammonia-N were highest with urea and lowest with soybean meal. The urea ration was associated with a significantly lower (P< 0.01) total VFA concentration and more alkaline pH.