Characterization of the Naturally Occurring Oxacillinase ofAcinetobacter baumannii
Open Access
- 1 October 2005
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 49 (10) , 4174-4179
- https://doi.org/10.1128/aac.49.10.4174-4179.2005
Abstract
A chromosomally encoded oxacillinase, OXA-69, was characterized from Acinetobacter baumannii AYE. β-Lactamase OXA-69 shared 97% amino acid identity with the recently described OXA-51 enzyme of A. baumannii and 62 and 56% amino acid identity with the carbapenem-hydrolyzing oxacillinases OXA-24 and OXA-23, respectively. Biochemical characterization of the purified OXA-69 revealed a narrow-spectrum hydrolysis profile but including, at a low level, imipenem and meropenem. By PCR and sequencing blaOXA-69-like genes were identified in all A. baumannii strains tested (n = 12), suggesting that this oxacillinase is naturally occurring in that species.Keywords
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