DIE JODFRAKTIONEN UND JODVERBINDUNGEN DER GESUNDEN MENSCHLICHEN SCHILDDRÜSE

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Abstract
After labelling in vivo, 14 normal and, as pilot experiments, 4 pathological human thyroids were studied for chemical iodine fractions (PBI, BEI and inorganic iodide) and for the individual iodinated compounds by means of paper and column chromatog-raphy. The best results of enzymatic hydrolysis were obtained by the combination of trypsin and pancreatin, which split off 80-95% of the iodine proteins. 16% of the thyroid iodine was found to be present in soluble form, 84% in the thyroglobulin complex and other proteins. Both fractions contained iodide, monoiodotyrosine, di-iodotyrosine, 3,5,3[image]-triiodothyronine, thyroxine and an unidentified iodine compound of thyronine character, comprising 8.3-12.4%. It could be isolated only by column chromatography, migrated on paper in 2 systems with a Rf of 0.8 together with triiodothyronine and would most probably correspond to 3,3[image]-diiodothyronine or 3,3[image],5[image]-triiodothyronine. The protein bound fraction of thyroid iodine contained more iodotyrosines than the soluble fraction.