Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box.
- 1 October 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (19) , 7322-7326
- https://doi.org/10.1073/pnas.85.19.7322
Abstract
The expression of HLA class II genes is regulated by a series of cis-acting elements and trans-acting factors. Several cis-acting elements have been identified and have been termed the Z box, X box, Y box, octamer, and "TATA" box. The Y box contains an inverted CCAAT box. By probing a phage .lambda.gt11 library with double-stranded oligonucleotides, we have directly isolated a cDNA encoding a Y box-binding protein designated YB-1. YB-1 binding has an absolute requirment for the CCAAT box and relative specificity for the Y box. It has a Mr of 35,414, contain 18% basic residues, and contains putative nuclear localization signals. An inverse correlation of YB-1 and HLA-DR .beta. chain mRNA levels suggests that YB-1 is a negative regulatory factor.This publication has 31 references indexed in Scilit:
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