DNA heterogeneity and phosphorylation unveiled by single-molecule electrophoresis
- 14 September 2004
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 101 (37) , 13472-13477
- https://doi.org/10.1073/pnas.0405568101
Abstract
Broad-spectrum analysis of DNA and RNA samples is of increasing importance in the growing field of biotechnology. We show that nanopore measurements may be used to assess the purity, phosphorylation state, and chemical integrity of nucleic acid preparations. In contrast with gel electrophoresis and mass spectrometry, an unprecedented dynamic range of DNA sizes and concentrations can be evaluated in a single data acquisition process that spans minutes. Because the molecule information is quantized and digitally recorded with single-molecule resolution, the sensitivity of the system can be adjusted in real time to detect trace amounts of a particular DNA species.Keywords
This publication has 12 references indexed in Scilit:
- Theory of DNA electrophoresis (∼ 1999 –2002 ½)Electrophoresis, 2002
- Stochastic sensors inspired by biologyNature, 2001
- Sequence-specific detection of individual DNA strands using engineered nanoporesNature Biotechnology, 2001
- Microsecond Time-Scale Discrimination Among Polycytidylic Acid, Polyadenylic Acid, and Polyuridylic Acid as Homopolymers or as Segments Within Single RNA MoleculesPublished by Elsevier ,1999
- Labeling Efficiency of Oligonucleotides by T4 Polynucleotide Kinase Depends on 5′-NucleotideAnalytical Biochemistry, 1998
- Structure of Staphylococcal α-Hemolysin, a Heptameric Transmembrane PoreScience, 1996
- Characterization of individual polynucleotide molecules using a membrane channelProceedings of the National Academy of Sciences, 1996
- Monte Carlo description of oligoelectrolyte properties of DNA oligomers: range of the end effect and the approach of molecular and thermodynamic properties to the polyelectrolyte limits.Proceedings of the National Academy of Sciences, 1989
- A new force field for molecular mechanical simulation of nucleic acids and proteinsJournal of the American Chemical Society, 1984
- SINGLE-STRANDED OLIGOMERS AND POLYMERS OF CYTIDYLIC AND 2′-DEOXYCYTIDYLIC ACIDS: COMPARATIVE OPTICAL ROTATORY STUDIESProceedings of the National Academy of Sciences, 1967