The expression of I-Ed molecules in F1 hybrid mice detected with antigen-specific, I-Ed-restricted cloned T-cell lines

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Abstract
The expression of polymorphic determinants on I-E molecules is largely dependent on allelic variation in the E β chain. We have previously analyzed the expression of E β k and E β b chains in F1 hybrid mice by a combination of techniques, and have shown that functional variation detected by the responsiveness of cloned T-cell lines specific for these molecules correlates well with serological determination of E β expression. In the present study, we have extended our analysis to E β d expression in F1 hybrid mice. We show that E β d is relatively poorly expressed in three F1 combinations: H-2d× H-2b, H-2d× H-2s, and H-2d× H-2u. The former two crosses express E α chains from the H-2dparent only; when recombinant strains carrying E β b or E β s and an active E α gene are used, E β d expression is significantly increased. On the other hand, H-2umice synthesize E α chains; the poor expression of E β d chains in this F1 hybrid apparently reflects the strong preferential association of E β u chains with all E α molecules thus far analyzed. These results confirm that E β chains compete for binding to E α chains and that preferential association of different allelic forms of E β chains with E α chains is a generalized phenomenon. They also illustrate the importance of the rate of biosynthesis of Ia chains for cell-surface expression.

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