A new and versatile reagent for incorporating multiple primary aliphatic amines into synthetic oligonucleotides
Open Access
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (18) , 7179-7186
- https://doi.org/10.1093/nar/17.18.7179
Abstract
A novel and versatile phosphorarnidite, N-Fmoc-O1-DMT-O2-cyanoethoxydiiopropylamino-phosphinyl-3-amino-1,2-propanediol (1, Fig. 1), has been synthesized and used to incorporate primary aliphatic amines into synthetic oligonucleotides. Its convenient preparation and use in solid phase oligonucleotide synthesis is described. Using phosphoramidite 1, an amino-modified oligonucleotide probe complementary to M13mp18 DNA was constructed with five primary amines attached to the 5′-terminus. The amino-modified oligonucleotide was subsequently labeled with biotin and employed in a dot-blot hybridization assay. As little as 0.5 ng of M13mp18 target DNA was colorimetrically detectedThis publication has 15 references indexed in Scilit:
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