Some characteristics of green-fluorescent pigment-producing bacteria

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Abstract
A study of 199 green-fluorescent bacteria is reported. By appropriate culture, several characteristics were found to be common to all cultures. These were production of green-fluorescent pigment, rod form, polar flagella, gram-negative staining, obligate aerobic relation to oxygen, failure to form indole, production of ammonia, growth at 20[degree] and 30[degree]C, death at 60[degree]C for 30 min., and a weak attack on carbohydrates. The cultures were divided into 14 groups on the basis of ability to grow at 5[degree] and 42[degree]C, action on milk, liquefaction of gelatin, and reduction of nitrates. Subgroups were established by the ability of the cultures to produce pyocyanin and to utilize sucrose, acetic acid, lactic acid, or tartaric acid as the sole carbon source. Growth of the cultures in different concs. of phenol, NaCN and NaCl is reported, along with growth in and hydrolysis of fat and action on nitrates when grown in a synthetic medium. The uniformity of the reduction of nitrates to N2 or gaseous N compounds and discrepancies in the reduction to nitrites are reported. Where possible, names of spp. most nearly describing the groups in this report are used. Group I (Pseudomonas aeruginosa) is best defined. For accurate definition, limiting temps. of growth must be used.