Acid Hydrolases in Normal B and T Blood Lymphocytes

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Abstract
B and T lymphocytes were separated by means of the spontaneous sheep red blood cell rosette formation technique from 3 normal donors. The following acid hydrolases were biochemically determined on separated B and T lymphocytes: acid phosphatase, β-glucuronidase, β-galactosidase, β-hexosaminidase, α-arabinosidase, α-galactosidase, α-mannosidase, α-glucosidase, and pH 4.0 and pH 5.0 β-glucosidase. The activities of most of the acid hydrolases including acid phosphatase and β-glucuronidase were found to be slightly decreased in B lymphocytes when compared to T lymphocytes. However, α-mannosidase activity was found to be significantly higher in the B lymphocytes than in the T lymphocytes and offers the possibility of using this enzyme as a B lymphocyte marker.