Regulation of 25 Hydroxyvitamin D31-Hydroxylase in Serum-Free Monolayer Culture of Mouse Kidney
- 1 March 1982
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 110 (3) , 1073-1075
- https://doi.org/10.1210/endo-110-3-1073
Abstract
Primary monolayer cultures of cells from normal mouse kidney were initiated and maintained in serum-free medium supplemented with insulin, transferrin and prostaglandin E1. Renal epithelial cells grow to confluence without detectable growth of fibroblasts. These cells contain an active 25(OH)D 3 1-hydroxylase with half-maximal formation of 1,25(OH>2D3 achieved at a substrate concentration of 13.3 nM. Activity of this enzyme is increased by low calcium medium or parathyroid hormone and decreased by high calcium medium, high phosphate medium or 1,25(HO)2D3Keywords
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