Genetic analysis of human B cell hybridomas expressing a cross-reactive idiotype.
Open Access
- 1 February 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 138 (3) , 940-944
- https://doi.org/10.4049/jimmunol.138.3.940
Abstract
We have examined the genetic basis for the expression of a human cross-reactive idiotype (CRI) commonly found on monoclonal IgM rheumatoid factors. The CRI was identified with a monoclonal antibody (17.109) and has been localized previously to the kappa-variable region. By using the human lymphoblastoid cell line WI-L2-729-HF2, and mononuclear cells from several sources, a panel of hybridomas was generated that produced 17.109 CRI-positive Ig. A recently cloned human germ-line V kappa III gene, Humkv305, served as a probe to identify genes which were rearranged and expressed in 17.109 CRI-positive and -negative hybridomas. This probe, when hybridized to human genomic DNA under stringent conditions, identified only two to five germ-line bands. In 10 separate 17.109 CRI-positive hybridoma clones, an additional rearranged V kappa band was identified. The probe did not anneal to rearranged V kappa bands in hybridoma clones that produced kappa-chains lacking the CRI. RNA dot-blot studies provided evidence for expression of genes hybridizing to the Humkv305 probe. The results indicate that the 17.109 CRI is a serologic marker for a single V kappa gene, or a small family of closely related V kappa genes, which is identified by the Humkv305 probe.This publication has 18 references indexed in Scilit:
- Delineation of a cross-reactive idiotype on human autoantibodies with antibody against a synthetic peptide.The Journal of Experimental Medicine, 1984
- Isolation of hybridomas expressing a specific heavy chain variable region gene segment by using a screening technique that detects mRNA sequences in whole cell lysates.Proceedings of the National Academy of Sciences, 1984
- A common idiotope on human rheumatoid factors identified by a hybridoma antibodyMolecular Immunology, 1983
- Preferential association of kappa IIIb light chains with monoclonal human IgM kappa autoantibodies.The Journal of Immunology, 1983
- The genetic basis of antibody production: A single heavy chain variable region gene encodes all molecules bearing the dominant anti‐arsonate idiotype in the strain A mouseEuropean Journal of Immunology, 1982
- A single VH gene segment encodes the immune response to phosphorylcholine: Somatic mutation is correlated with the class of the antibodyCell, 1981
- Complete amino acid sequence of variable domains from two monoclonal human anti-gamma globulins of the Wa cross-idiotypic group: suggestion that the J segments are involved in the structural correlate of the idiotype.Proceedings of the National Academy of Sciences, 1981
- Human Vκ immunoglobulin gene number: Implications for the origin of antibody diversityCell, 1981
- Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segmentsCell, 1980
- PROTEIN-A OF STAPHYLOCOCCUS-AUREUS IS MITOGENIC FOR IGG-BEARING, BUT ALSO FOR A SUB-POPULATION OF IGM-BEARING AND-OR IGD-BEARING HUMAN-LYMPHOCYTES1980