CHLAMYDOSPORULATION IN CANDIDA-ALBICANS - INFLUENCE OF SOWING DENSITY AND GLUCOSE, NITROGEN, BIOTIN AND MINERAL SALTS CONCENTRATION IN RICE-CREAM DECOCTION

  • 1 January 1978
    • journal article
    • research article
    • Vol. B129  (2) , 183-193
Abstract
Chlamydospore production by C. albicans was investigated in rice cream decoction inoculated with a blastospore suspension. Effects of glucose, KH2PO4, (NH4)2SO4 and biotin addition were estimated using a chlamydospore index, i.e., the mean number of mature chlamydospores produced from a single blastospore development. If no other component was added, it appeared that better sporulation was obtained with both glucose concentration and inoculum size optimum (0.5 g/l and 7.8 .times. 103 blastospores/ml, respectively). In glucose-KH2PO4 enriched medium, chlamydosporulation was best with 0.5 g/l glucose and 0.5 g/l KH2PO4. The addition of biotin reduced the chlamydospore index at the concentrations tested (0.6 to 20 .mu.g/l). When glucose and (NH4)2SO4 were added together, it seemed that one of the C or N component had to be in low concentration for good sporulation. The action of salts was also investigated. A synthetic medium is proposed; the improvement of this medium is now in progress.