Germline Organization of the Murine T Cell Receptor β-Chain Genes
- 23 October 1987
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 238 (4826) , 545-548
- https://doi.org/10.1126/science.2821625
Abstract
The complete germline organization of the beta-chain genes of the murine T cell receptor was elucidated in order to obtain the structural basis for understanding the mechanisms of somatic DNA rearrangements. Twenty of the 22 known variable (V beta) genes are clustered within 250 kilobases of DNA 5' to the constant region (C beta) genes. These V beta genes share the same transcriptional orientation as the diversity (D beta), joining (J beta), and C beta genes, which implies that chromosomal deletion is the mechanism for most V beta to D beta-J beta rearrangements. Within this V beta cluster, the distance between the most proximal V beta gene and the D beta-J beta-C beta cluster is 320 kilobases, as determined by field-inversion gel electrophoresis. The large distance between V beta and D beta, relative to that between D beta and J beta, may have significant implications for the ordered rearrangement of the T cell receptor beta-chain genes.This publication has 40 references indexed in Scilit:
- A T cell receptor Vβ segment that imparts reactivity to a class II major histocompatibility complex productCell, 1987
- T cell-specific gamma genes in C57BL/10 mice. Sequence and expression of new constant and variable region genes.The Journal of Experimental Medicine, 1986
- Direct evidence for chromosomal inversion during T-cell receptor β-gene rearrangementsNature, 1986
- Introduced T cell receptor variable region gene segments recombine in pre-B cells: Evidence that B and T cells use a common recombinaseCell, 1986
- T-Cell Receptor β-Chain Expression: Dependence on Relatively Few Variable Region GenesScience, 1985
- Rearranged β t cell receptor genes in a helper t cell clone specific for lysozyme: No correlation between Vβ and MHC restrictionCell, 1985
- Novel immunoglobulin heavy chains are produced from DJH gene segment rearrangements in lymphoid cellsNature, 1984
- Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell linesNature, 1984
- Identification of a diversity segment of human T-cell receptor β-chain, and comparison with the analogous murine elementNature, 1984
- Somatic generation of antibody diversityNature, 1983