Functional Integrity of Cryopreserved Human Monocytes23
- 1 February 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 66 (2) , 255-260
- https://doi.org/10.1093/jnci/66.2.255
Abstract
Human peripheral blood monocytes collected by semicontinuous flow centrifugation were cryopreserved in varying concentrations of dimethyl sulfoxide (DMSO). Optimum protection from lethal cryoinjury was achieved with 7.5–10% DMSO. Compared to fresh cells. cryopreserved cells retained 80% of their chemotactic responsiveness and fructose-6-phosphate shunt activity after stimulation by zymosan. Fresh and cryopreserved human monocytes had comparable but low levels of cytotoxic activity against P-815 murine mastocytoma cells in vitro.Keywords
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