Sequence analysis of a cDNA encoding a Group 3 LEA mRNA inducible by ABA or dehydration stress in wheat
- 1 June 1991
- journal article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 16 (6) , 1073-1076
- https://doi.org/10.1007/bf00016078
Abstract
A cDNA clone (pMA2005) of a Group 3 LEA (late embryogenesis abundant) protein has been sequenced from wheat. The wheat cDNA clone codes for a protein with ten tandem repeats of an 11 amino acid sequence and has homology to other Group 3 LEAs reported in barley, carrot, cotton and rape (L. Dure et al., Plant Mol Biol 12: 475–486, 1989). The deduced amino acid sequence indicates that the wheat protein has a molecular weight of 23 000 and is a basic, hydrophilic protein. Northern analysis with the cDNA clone shows that dehydration of wheat shoot tissue results in increased transcript levels that correlate with increases in endogenous ABA.Keywords
This publication has 9 references indexed in Scilit:
- Molecular Cloning and Expression of Abscisic Acid-Responsive Genes in Embryos of Dormant Wheat SeedsPlant Physiology, 1991
- A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and cornPlant Molecular Biology, 1989
- Reduced Accumulation of ABA during Water Stress in a Molybdenum Cofactor Mutant of BarleyPlant Physiology, 1989
- Common amino acid sequence domains among the LEA proteins of higher plantsPlant Molecular Biology, 1989
- A simple, rapid method for the purification of poly A+ RNA.1988
- Cloning and characterization of a cDNA encoding a mRNA rapidly-induced by ABA in barley aleurone layersPlant Molecular Biology, 1988
- The nucleotide sequence of a cDNA clone encoding the wheat EmproteinNucleic Acids Research, 1987
- A comprehensive set of sequence analysis programs for the VAXNucleic Acids Research, 1984
- Developmental biochemistry of cottonseed embryogenesis and germination: changing messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesisBiochemistry, 1981