MONOCLONAL IGG4 (LAMBDA) WITH FACTOR-V INHIBITORY ACTIVITY

  • 1 January 1979
    • journal article
    • research article
    • Vol. 122  (5) , 2119-2121
Abstract
A monoclonal Ig[immunoglobulin]G4 (.lambda.) with inhibitory activity to human coagulation factor V was isolated from the serum of a patient with a fatal hemorrhagic diathesis by using a combination of DE-52 ion exchange chromatography and isoelectric focusing techniques. Using the criteria for defining a monoclonal Ig of restricted mobility on protein electrophoresis, immunoelectrophoresis and isoelectric focusing, and neutralization with class, subclass and L chain type antisera, was the 1st demonstration of a factor V inhibitor as a monoclonal IgG4 (.lambda.) detectable in serum or plasma.