CFU-S KINETICS OBSERVED INVIVO BY BROMODEOXYURIDINE AND NEAR-UV LIGHT TREATMENT

  • 1 January 1981
    • journal article
    • research article
    • Vol. 9  (2) , 123-128
Abstract
Using continuous BrdUrd [bromodeoxyuridine] infusion in vivo and near-UV light irradiation in vitro, the time integrated S-phase fraction of CFUS derived from murine bone marrow was measured. These measurements indicate a stochastic commitment to cycle of essentially the entire population of CFUS [pluripotential stem cells], consistent with the existence of a non-cycling cell state G0. For B6D2F1 female mice the CFUS are committed to DNA synthesis .apprx. once every 52 h. Measurements of other CFUS parameters showed no significant effects of the BrdUrd infusion. Hydroxyurea challenge of BrdUrd-infused mice indicates that the capacity of the CFUS to respond to a proliferative stimulus during the infusion period is unaffected by the BrdUrd.