Effect of Detergents on Streptolysin S Precursor

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Abstract
Group A streptococci which produce streptolysin S contain a cellular precursor to streptolysin S in the membranes and cytoplasm which is activatable by blending in a Vortex mixer with glass beads and RNA-core (RNA preparation from yeast). Although no activation of precursor occurred when it was mixed with detergents, it was activated when blended with glass beads and detergents such as Tergitol NP-40 and Brij 35. Maximum activation of precursor was achieved in 1-2% detergent, in pH 6.5 buffer and after 8 min of blending. Detergents Tween 20, 40, 60 and 80, Brij 56 and Lubrol WX also activated precursor, but, of all the hemolysin preparations, those with Tween 40 or 60 or Lubrol WX were the most stable. The addition of RNA-core during or after blending of precursor with detergents enhanced the titer and stability of the hemolysin. This was due in part to the association of the hemolytic moiety with RNA-core. Activation of precursor in the membrane was better with a detergent, while that in the cytoplasm was better with RNA-core. Precursor from 2 different cellular locations can be differentiated by the effects of RNA-core and detergents on precursor titer.