New plant binary vectors with selectable markers located proximal to the left T-DNA border
- 1 December 1992
- journal article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 20 (6) , 1195-1197
- https://doi.org/10.1007/bf00028908
Abstract
Five new binary vectors have been constructed which have the following features: (1) different plant selectable markers including neomycin phosphotransferase (nptII), hygromycin phosphotransferase (hpt), dihydrofolate reductase (dhfr), phosphinothricin acetyl transferase (bar), and bleomycin resistance (ble); (2) selectable markers are located near the T-DNA left border and; (3) selectable marker and β-glucuronidase (uidA) reporter genes are divergently organized for efficient expression, and can easily be removed or replaced as needed.Keywords
This publication has 14 references indexed in Scilit:
- Improved method for the transformation of Arabidopsis thaliana with chimeric dihydrofolate reductase constructs which confer methotrexate resistancePlant Cell Reports, 1992
- A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.Proceedings of the National Academy of Sciences, 1990
- Binary vectors which allow the exchange of plant selectable markers and reporter genesNucleic Acids Research, 1990
- A cassette containing thebargene ofStreptomyces hygroscopicus: a selectable marker for plant transformationNucleic Acids Research, 1990
- Uptake and transient expression of chimeric genes in seed-derived embryos.Plant Cell, 1989
- BASIC PROCESSES UNDERLYING AGROBACTERIUM-MEDIATED DNA TRANSFER TO PLANT CELLSAnnual Review of Genetics, 1988
- GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.The EMBO Journal, 1987
- Engineering herbicide resistance in plants by expression of a detoxifying enzymeThe EMBO Journal, 1987
- Bleomycin resistance: a new dominant selectable marker for plant cell transformationPlant Molecular Biology, 1986
- BinaryAgrobacteriumvectors for plant transformationNucleic Acids Research, 1984