The phorbol ester-induced extracellular Ca2+ -independent release of LH is dependent on estradiol and de novo protein synthesis

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Abstract
Phorbol 12-myristate 13-acetate (PMA) was used to determine whether the PMA-induced extracellular Ca2+-independent release of LH was dependent on sex, estradiol and de novo protein synthesis. Infusions of gonadotropin-releasing hormone (GnRH) or PMA in a perifusion system stimulated a partial secretion of LH from diestrous II and ovariectomized + estradiol-treated female pituitaries (responses inhibited by cycloheximide). In contrast, PMA was ineffective in stimulating PRL secretion from these pituitaries, as well as LH secretion from male or ovariectomized female pituitaries. These results indicate that the PMA-stimulated extracellular Ca2+-independent secretion of LH is a specific process which is dependent on sex, estradiol and de novo protein synthesis, and mimics the characteristics of the GnRH-stimulated responses.