An Hsp60 related protein is associated with purified HIV and SIV
- 5 February 1994
- journal article
- Published by Wiley in Journal of Medical Primatology
- Vol. 23 (2-3) , 151-154
- https://doi.org/10.1111/j.1600-0684.1994.tb00116.x
Abstract
The possible physical association of heat shock proteins (Hsp's) with immunodeficiency viruses (HIV and SIV) has been examined. The virions were purified by a) polyethylene glycol (PEG) precipitation and Sepharose 4B filtration, b) PEG precipitation and centrifugation over a Renografin gradient, or c) PEG precipitation and Matrex Cellufine Sulfate affinity chromatography. Western blotting revealed an Hsp60 related protein associated with HIV and SIV. Other Hsp's (such as Hsp70) were not detected, suggesting a specific interaction between Hsp60 and viral factors.Keywords
This publication has 45 references indexed in Scilit:
- Human Hsp60: bacterial expression, purification, development of monoclonal antibodies and a sandwich ELISA for quantitationImmunology Letters, 1994
- Protein folding in the cellNature, 1992
- THE UNIVERSALLY CONSERVED GroE (Hsp60) CHAPERONINSAnnual Review of Microbiology, 1991
- Heat shock proteins and the immune responseImmunology Today, 1990
- Heat Shock Is Lethal to Fibroblasts Microinjected with Antibodies Against hsp70Science, 1988
- Nonrandom association of cellular antigens with HTLV-III virionsHuman Immunology, 1987
- Isolation of T-Cell Tropic HTLV-III-Like Retrovirus from MacaquesScience, 1985
- The Heat Shock ResponsCritical Reviews in Biochemistry, 1985
- Molecular cloning of lymphadenopathy-associated virusNature, 1984
- Induction of the synthesis of a 70,000 dalton mammalian heat shock protein by the adenovirus E1A gene productCell, 1982