Double-stranded RNA of Rice Gall Dwarf Virus

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Abstract
Summary Nucleic acid extracted from purified particles of rice gall dwarf virus (RGDV) was identified as double-stranded RNA because it (i) was susceptible to RNase A in 0.1 × standard saline citrate (SSC) but not in 1 × SSC, (ii) was resistant to nucleae S1, (iii) showed no hyperchromicity in u.v. absorption after treatment with formaldehyde at 37 °C, (iv) showed a sharp thermal transition in u.v. absorption in 0.01 × SSC and (v) had a buoyant density of 1.596 g/ml in Cs2SO4. Electrophoresis in polyacrylamide slab gel revealed that RGDV RNA was composed of 12 segments with a total mol. wt. of about 16.9 × 106. Co-electrophoresis of RNA from RGDV and rice dwarf virus demonstrated that the electrophoretic mobilities of the seven larger segments from the two viruses were similar but that the five smaller segments differed in this respect. These results confirm that RGDV is a new virus and a third member of the genus Phytoreovirus.