Persistence of cyclophosphamide-induced damage in bone marrow as indicated by sister chromatid exchange analysis

31 December 1984

journal article
research article
Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research

Vol. 6 (8) , 1097-1102
https://doi.org/10.1093/carcin/6.8.1097

Abstract

Various treatment protocols were used to investigate the time-dependent decrease in sister chromatid exchanges (SCEs) in bone marrow cells following treatment of C57B1/6J .times. DBA/2J F1 (DB2F1) mice by i.p. injection of cyclophosphamide (15 mg/kg). The major factor in the time-related decrease in SCE-inducing lesions is the considerable cytotoxicity or selection of less highly damaged cells over successive cyclophosphamide post-treatment cycles. A constant rate of selection of 0.61% and 0.65%, respectively, was apparent over post-treatment cycles 1-2 and 2-3. In addition, 2nd and 3rd-division SCE data produced by various protocols indicate persistence of a fraction of cyclophosphamide''s SCE-inducing lesions for at least 3 post-treatment cycles. Comparison of the persistence of cyclophosphamide''s SCE-inducing lesions with previously reported data for diepoxybutane and ethyl carbamate reveals that the rate of repair of SCE-inducing lesions is inversely related to tumorigenic activities.

This publication has 19 references indexed in Scilit:

POTENCY OF SOME CARBAMATES AS MULTIPLE TISSUE SISTER CHROMATID EXCHANGE INDUCERS AND COMPARISON WITH KNOWN CARCINOGENIC ACTIVITIES
1981
Cytogenetic effects of inhaled benzene in murine bone marrow: induction of sister chromatid exchanges, chromosomal aberrations, and cellular proliferation inhibition in DBA/2 mice.
Proceedings of the National Academy of Sciences, 1980
Sister-chromatid exchanges and chromosal breakage in patients treated with cytostatics
Mutation Research/Genetic Toxicology, 1979
Sister chromatid exchange in murine alveolar macrophages, regenerating liver and bone marrow cells ? a simultaneous multicellular in vivo assay
Chromosoma, 1979
Further analysis of the replication bypass model for sister chromatid exchange
Human Genetics, 1979
Induction of long-lived chromosome damage, as manifested by sister-chromatid exchange, in lymphocytes of animals exposed to mitomycin-C
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
Sister chromatid exchange studies for monitoring DNA damage and repair capacity after cytostatics in vitro and in lymphocytes of leukaemic patients under cytostatic therapy
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
Comparisons of in vivo BrdU labeling methods and spontaneous sister chromatid exchange frequencies in regenerating murine liver and bone marrow cells
Chromosoma, 1978
In vivo analysis of cellular replication.
Proceedings of the National Academy of Sciences, 1977
In vivo BUdR labeling of mammalian chromosomes
Experimental Cell Research, 1976