Expression of a Bacterial Gene in Mammalian Cells
- 19 September 1980
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 209 (4463) , 1422-1427
- https://doi.org/10.1126/science.6251549
Abstract
Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells.Keywords
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