Structural Changes in Mammalian Cells Associated with Cooling to -79°C
Open Access
- 1 July 1960
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 7 (4) , 673-678
- https://doi.org/10.1083/jcb.7.4.673
Abstract
Suspensions of HeLa and S37 cells, with and without added glycerol, were cooled in stages to -79°C. and held at that temperature for 30 minutes. After warming to room temperature the cells were fixed, sectioned, and compared by phase contrast and electron microscopy with similar specimens kept at room temperature. Correlated viability tests were made.Keywords
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