BIOSYNTHESIS OF STEROIDS BY GRANULOSA CELLS OF THE PORCINE OVARY IN VITRO

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Abstract
Incubation of granulosa cell aggregates from porcine ovarian follicles with progesterone-4-14C, 17[alpha]-hydroxyprogesterone-4-14C, pregneno-lone-7[alpha]-3H, testosterone-3-14C and androstene-3,17-dione-4-14C showed that they contained efficient 3[beta] -hydroxysteroid dehydrogenase, 20[alpha]-hydroxysteroid dehydrogenase and 17[beta] -hydroxysteroid dehydrogenase enzyme activities. There were also some aromatizing and probably some 21-hydroxylating enzyme activities. The granulosa cells were particularly low in 17[alpha]-hydroxylase and sidechain cleaving enzyme activities although some activity was demonstrated.