Specific binding sites for a parvovirus, minute virus of mice, on cultured mouse cells

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Abstract
Early interactions between parvoviruses and host cells were not previously extensively described. In this study some aspects of viral binding to the cell surface were characterized and the existence of specific cellular receptor sites for minute virus of mice (MVM) on 2 murine cell lines that are permissive for viral growth were demonstrated. The interaction had a pH optimum of 7.0-7.2, and the rate and extent of the reactions were slightly affected by temperature. Mouse A-9 cells (L-cell derivative) had .apprx. 5 .times. 105 specific MVM binding sites/cell, and Friend erythroleukemia cells had 1.5 .times. 105 MVM sites/cell. The nonpermissive mouse lymphoid cell line L1210 lacked specific viral receptors. Cloned lines of A-9 cells resistant to viral infection were isolated. One of these lines lacked the specific virus attachment sites but exhibited low levels of nonsaturable virus binding. Based on these examples, infectivity is correlated with the presence of specific viral receptors on the cell surface.