Molecular Tripods Showing Fluorescence Enhancement upon Binding to Streptavidin
- 15 December 2004
- journal article
- Published by American Chemical Society (ACS) in Organic Letters
- Vol. 7 (1) , 111-114
- https://doi.org/10.1021/ol047801h
Abstract
We introduce a new approach to biotin-streptavidin assays based on a molecular tripod which consists of biotin, a fluorophore, and a quencher. The interaction between streptavidin and molecular tripods perturbs the ground-state quencher-fluorophore dimeric conformation in the absence of streptavidin and diminishes the intrinsic self-quenching of a quencher-fluorophore pair. The emission intensity of the molecular tripods plus streptavidin is 3.5-5.2 times that of molecular tripods in the absence of streptavidin.close131Keywords
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