Selection for temperature‐sensitive mutants of diploid and tetraploid mammalian cells

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Abstract
Mammalian cell populations may be enriched for temperature‐sensitive (ts) mutants by the tritiated thymidine (3H‐TdR) suicide procedure (Thompson et al., '71). Such procedures were carried out on the near‐diploid Chinese hamster ovary (CHO) cell line and on a line made “tetraploid” by Colcemid treatment. Clones of ts mutants were obtained from the diploid line, but in spite of repeated attempts, no ts mutants were isolated from the tetroploid line. The phenotypes of the new diploid ts mutants recovered were consistent with the particular selection regime employed.