Expression and electrophysiological identification of the receptor for bombesin and gastrin‐releasing peptide in Xenopus laevis oocytes injected with polyA+ RNA from rat brain

Abstract
The receptor for bombesin and the related peptide, gastrin‐releasing peptide (GRP) has been induced in frog oocytes by injection of polyA+ RNA from rat brain. The primed oocytes responded to peptides of the bombesin family (GRP, neuromedin C of bombesin) by showing dose‐dependent oscillations in membrane currents as recorded by the voltage‐clamp method. The induced membrane changes were suppressed when oocytes were pretreated with a bombesin‐receptor antagonist.