Differentially expressed genes associated with CIS‐diamminedichloroplatinum (II) resistance in head and neck cancer using differential display and CDNA microarray

Publisher Website
Google Scholar
Abstract
Background. The mechanism by which cancer cells become resistant to cis‐Diamminedichloroplatinum (II) (cDDP) is not completely understood. To investigate the molecular markers involved in the cDDP resistance, we compared the gene expression profiles between a head and neck squamous cell carcinoma (HNSCC) line sensitive to cDDP and its cDDP‐resistant variant. Methods. Both a fluorescent differential display and a cDNA microarray analysis were applied to distinguish the gene profiles between KB, a human HNSCC line, and its cDDP‐resistant variant (KB/cDDP). These results were confirmed by Northern blot analysis. Results. One up‐regulated gene, glycoprotein hormone α‐subunit, and two down‐regulated genes coding membrane proteins, human folate receptor and tumor‐associated antigen L6, were identified in KB/cDDP cells. Conclusions. Our findings suggest that development of the cDDP‐resistant phenotype is accompanied by alternations of gene expression including a glycoprotein hormone and membrane proteins. These gene products could be new molecular markers for resistance to cDDP. © 2003 Wiley Periodicals, Inc. Head Neck 25: 187–193, 2003