Mechanism of UDP‐sugar transport into intracellular vesicles
- 2 November 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 208 (2) , 407-412
- https://doi.org/10.1016/0014-5793(86)81058-4
Abstract
The mechanism of translocation of UDP‐GlcNAc, UDP‐Gal and UDP‐Glc into intracellular vesicles has been studied using thymocytes whose plasma membranes have been permeabilized with isotonic ammonium chloride. It has been previously shown that the intracellular vesicles have specific carriers for UDP‐GlcNAc and UDP‐Gal. We now report that the translocation of these two sugar nucleotides occurs via UDP‐GlcNAc/UDP and UDP‐Gal/UDP antiports. The entry of UDP‐GlcNAc or UDP‐Gal into vesicles was specifically dependent on the exit of UDP from these vesicles. In contrast, no antiport mechanism has been recovered with UDP‐Glc for which no transport and accumulation into intracellular vesicles were observed.Keywords
This publication has 17 references indexed in Scilit:
- Dilatation of Golgi vesicles by monensin leads to enhanced accumulation of sugar nucleotidesBioscience Reports, 1986
- Accumulation of UDP‐GlcNAc into intracellular vesicles and occurrence of a carrier‐mediated transportEuropean Journal of Biochemistry, 1985
- Intralumenal pool and transport of CMP-N-acetylneuraminic acid, GDP-fucose and UDP-galactose. Study with plasma-membrane-permeabilized mouse thymocytesBiochemical Journal, 1984
- Mechanisms of glycosylation and sulfation in the Golgi apparatus: evidence for nucleotide sugar/nucleoside monophosphate and nucleotide sulfate/nucleoside monophosphate antiports in the Golgi apparatus membrane.Proceedings of the National Academy of Sciences, 1984
- Evidence for coupling between transport of UDP-glucose and its synthesis by membrane-bound pyrophosphorylase in golgi apparatus of cat liverBiochimica et Biophysica Acta (BBA) - Biomembranes, 1984
- Glycosylation of proteins from sugar nucleotides by whole cells. Effect of ammonium chloride treatment on mouse thymocytesBiochemical Journal, 1983
- Evidence for the sorting of endocytic vesicle contents during the receptor-mediated transport of IgG across the newborn rat intestine.The Journal of cell biology, 1981
- Orientation of glycoprotein galactosyltransferase and sialyltransferase enzymes in vesicles derived from rat liver Golgi apparatus.The Journal of cell biology, 1981
- Translocation of cytidine 5′-monophosphosialic acid across golgi apparatus membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1981
- The kinetics of iron uptake in vitro by human duodenal mucosa: studies in normal subjects.The Journal of Physiology, 1979